Plasmid_Backbone

Part:BBa_K1795023:Design

Designed by: Andrew Halleran, Joseph Maniaci, Caroline Golino, Taylor Jacobs, John Marken, Elli Cryan, Panya Vij, Michael LeFew   Group: iGEM15_William_and_Mary   (2015-09-18)


GalK Integrator Cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2078
    Illegal suffix found at 2100
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2078
    Illegal SpeI site found at 2101
    Illegal PstI site found at 2115
    Illegal NotI site found at 2084
    Illegal NotI site found at 2108
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2078
    Illegal XhoI site found at 1012
    Illegal XhoI site found at 1904
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2078
    Illegal suffix found at 2101
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2078
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2093
    Illegal SpeI site found at 2101
    Illegal PstI site found at 2115
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 2069


Design Notes

Source

iGEM Parts Registry. Homologous regions were modeled after those used in Elowitz, M. B., Levine, A. J., Siggia, E. D., & Swain, P. S. (2002). Stochastic gene expression in a single cell. Science, 297(5584), 1183-1186.

References